ABCD4 Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS5595-50, BS5595-100
Sizes: 50µl, 100µl
Swiss-Prot: O14678
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: The peroxisomal membrane contains several ATP-binding cassette (ABC) transporters, ABCD1-4 that are known to be present in the human peroxisome membrane. All four proteins are ABC half-transporters, which dimerize to form an active transporter. A mutation in the ABCD1 gene causes X-linked adreno-leukodystrophy (X-ALD), a peroxisomal disorder which affects lipid storage. ABCD2 in mouse is expressed at high levels in the brain and adrenal organs, which are adversely affected in X-ALD. The peroxisomal membrane comprises two quantitatively major proteins, PMP22 and ABCD3. ABCD3 is associated with irregularly shaped vesicles which may be defective peroxisomes or peroxisome precursors. ABCD1 localizes to peroxisomes. ABCB7 is a half-transporter involved in the transport of heme from the mitochondria to the cytosol.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: ABCD4 polyclonal antibody detects endogenous levels of ABCD4 protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ABCD4 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Rat kidney tissue lysate, Immunohistochemistry (IHC) analyzes of ABCD4 pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing peroxisome membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~ 73 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: ATP-binding cassette sub-family D member 4; PMP70-related protein; P70R; Peroxisomal membrane protein 1-like; PXMP1-L; Peroxisomal membrane protein 69; PMP69; ABCD4; PXMP1L
Immunogen: Synthetic peptide, corresponding to amino acids 157-202 of Human ABCD4.
Conjugate: Unconjugated
Modification: Unmodified