ABCF1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90013-50, BS90013-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q8NE71(Human) Q6MG08(Rat)
Host: Rabbit
Reactivity: Human, Rat
Applications: WB, ICC/IF, IHC
All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
Background: ABCF1 (ATP-binding cassette sub-family F member 1, TNF-alpha-stimulated ABC protein) is a 845 amino acid protein encoded by the human gene ABCF1. ABCF1 belongs to the ABC transporter family (EF3 subfamily) and contains two ABC transporter domains. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. ABCF1 is believed to have a role in mRNA translation due to its interaction with eukaryotic initiation factor 2 (eIF2). It is also associated with ribosomes. ABCF1 is ubiquitously expressed and can be induced with TNF. Upon DNA damage, ABCF1 is phosphorylated by either ATM or ATR.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: ABCF1 polyclonal antibody detects endogenous levels of ABCF1 protein.
Bioworld Molecular Weight: 96 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of ABCF1 on Hela lysates using anti-ABCF1 antibody at 1/1,000 dilution., ICC staining ABCF1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: ABCF1, ABC27, ABC50, ABCF1, ABCF1, ATP binding cassette 50 (TNF alpha stimulated), ATP binding cassette 50, ATP binding cassette sub family F member 1, ATP binding cassette, sub family F (GCN20), member 1, ATP-binding cassette 50, ATP-binding cassette sub-family F member 1, AU041969, D17Wsu166e, EST123147, GCN20, TNF alpha stimulated ABC protein, TNF-alpha-stimulated ABC protein, TNFalpha inducible ATP binding protein, wu:fb79c06, wu:fc39a05, wu:fj94a08, zgc:85667,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified