ABHD13 Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS60600-50, BS60600-100
Sizes: 50µl, 100µl
Swiss-Prot: Q7L211
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: The α/β hydrolase superfamily comprise diverse members that are involved in important biochemical processes and related to various diseases. They have unrelated sequences, various substrates, and different kinds of catalytic activities, yet they share the same canonical å/β hydrolase fold, which consists of an eightstranded parallel å/β structure. They are also characterized by a catalytic triad composed of a histidine, an acid and a nucleophile. Members of this superfamily are often drug targets for treating diseases, such as diabetes, Alzheimer’s disease, obesity and blood clotting disorders. The Ab hydrolase domain containing (ABHD) gene subfamily is comprised of 15 mostly uncharacterized members. Most of which utilize a serine nucleophile to form the G-X-S-X-G nucleophile elbow. ABHD1 plays a role in metabolizing smoking xenobiotics. ABHD2 participates in the development of atherosclerosis. ABHD4 is involved in an alternative synthesis pathway of NAE. Mutations in ABHD5 contribute to Chanarin-Dorfman syndrome. ABDH6 may play a role in nervous system metabolism and signaling. ABHD13 is a 337 amino acid single-pass membrane protein that belongs to the ABHD family.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: ABHD13 polyclonal antibody detects endogenous levels of ABHD13 protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ABHD13 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate , Immunohistochemistry (IHC) analyzes of ABHD13 pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~ 39 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: Alpha/beta hydrolase domain-containing protein 13; Abhydrolase domain-containing protein 13; ABHD13; C13orf6
Immunogen: A synthetic peptide corresponding to residues in Human ABHD13
Conjugate: Unconjugated
Modification: Unmodified