ABHD4 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS61120-50, BS61120-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q8TB40
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
Background: The α/β hydrolase superfamily is comprised of diverse members that are involved in important biochemical processes and related to various diseases. They have unrelated sequences, various substrates, and different kinds of catalytic activities, yet they share the same canonical α/β hydrolase fold, which consists of an eight-stranded parallel α/β structure. They are also characterized by a catalytic triad composed of a histidine, an acid and a nucleophile. Members of this superfamily are often drug targets for treating diseases, such as diabetes, Alzheimer’s disease, obesity and blood clotting disorders. ABHD1 plays a role in metabolizing smoking xenobiotics. ABHD2 participates in the development of atherosclerosis. ABHD4 is involved in an alternative synthesis pathway of NAE. ABHD4 is a lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE) which participates in the biosynthesis of N-acyl ethanolamines. Mutations in ABHD5 contribute to Chanarin-Dorfman syndrome. ABDH6 may play a role in nervous system metabolism and signaling. ABHD14A is possibly involved in granule neuron development.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ABHD4 polyclonal antibody detects endogenous levels of ABHD4 protein.
Bioworld Molecular Weight: ~ 39 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ABHD4 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: RAW264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of ABHD4 pAb in paraffin-embedded mouse brain carcinoma tissue at 1:50, showing cytoplasmic and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ABHD4 pAb in paraffin-embedded human rectum carcinoma tissue at 1:50, showing cytoplasmic and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: Abhydrolase domain-containing protein 4 (EC:3.1.1.-); Alpha/beta-hydrolase 4; Lyso-N-acylphosphatidylethanolamine lipase; ABHD4
Immunogen: A synthetic peptide corresponding to residues in Human ABHD4.
Conjugate: Unconjugated
Modification: Unmodified