Product Description

ACCα (phospho-S80) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4210-50, BS4210-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Acetyl-CoA carboxylase (ACC) is a complex mμltifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the ratelimiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl- CoA carboxylase in human muscle. ACCα (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACCβ (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACCα is regμlated by phosphorylation (inactive) and dephosphorylation (active) of targeted Serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the short-term regμlatory mechanism of the enzyme. The gene encoding ACCα, which maps to human chromosome 17, encodes the 265 kDa α form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACCβ is homologous to that of ACCα except for an additional peptide of about 150 amino acids at the N-terminus.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Acetyl-CoA carboxylase 1, Short name=ACC1, ACC-alpha, Biotin carboxylase, ACACA, ACAC, ACC1, ACCA

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human ACCα around the phosphorylation site of Serine 80.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-ACCα (S80) polyclonal antibody etects endogenous levels of ACCα protein only when phosphorylated at Ser80.

Molecular Weight: ~ 265 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13085

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only