ACER2 Polyclonal Antibody
Catalogue Numbers: BS60828-50, BS60828-100
Sizes: 50µl, 100µl
Alternative Name: Alkaline ceramidase 2; AlkCDase 2; Alkaline CDase 2; haCER2; Acylsphingosine deacylase 3-like; N-acylsphingosine amidohydrolase 3-like; ACER2; ASAH3L; PP11646
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q5QJU3
Host: Rabbit
Reactivity: Human, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ACER2 polyclonal antibody detects endogenous levels of ACER2 protein.
Bioworld Molecular Weight: ~ 31 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ACER2 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human ACER2.
Conjugate: Unconjugated
Modification: Unmodified