AChRα1 (E217) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS2850-50, BS2850-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P02708
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
Background: Nicotinic acetylcholine receptors(AChRs) are found at the postsynaptic membrane of the neuromuscular junctionand bind acetylcholine molecules, allowing ions to move through thepore. Glutamate receptors are found in the postsynaptic membrane of cellsin the central nervous system. AChRα1, also known as ACHRD, CHRNA, CMS2A, FCCMS, SCCMS or CHRNA1, is a 482 amino acid multi-pass membrane protein that exists as two alternatively spliced isoforms, which are expressed in different tissues. Isoform 1 is only expressed in skeletal muscle whereas isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: AChRα1 (E217) polyclonal antibody detects endogenous levels of AChRα1 protein.
Bioworld Molecular Weight: ~ 55 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of AChRα1 (E217) pAb at 1:500 dilution
Lane 1: HepG2 whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug)
Lane 3: PC3 whole cell lysate (40ug)
Lane 4: CT26 whole cell lysate (40ug)
Lane 5: H9C2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of AChRα1 (E217) pAb bin paraffin-embedded human liver carcinoma tissue at 1:50, showing membrane and cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of AChRα1 (E217) pAb bin paraffin-embedded human kidney carcinoma tissue at 1:50, showing membrane and cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: Acetylcholine receptor subunit alpha; ACHRA; CHNRA; CHRNA1; ACHRD; CMS2A; FCCMS, SCCMS; Acra
Immunogen: Synthetic peptide, corresponding to amino acids 181-230 of Human AChRα1.
Conjugate: Unconjugated
Modification: Unmodified