ACSL5 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS8962-50, BS8962-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9ULC5
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 76kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western Blot analysis of extracts of various cell lines, using ACSL5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Alternative Name: ACSL5; ACS2; ACS5; FACL5
Immunogen: Recombinant fusion protein of human ACSL5(NP_057318.2).
Conjugate: Unconjugated
Modification: Unmodified