ACSS2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS8244-50, BS8244-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9NR19
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 78kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using ACSS2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Confocal immunofluorescence analysis of NIH-3T3 cells using ACSS2 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH-3T3 cells using ACSS2 Rabbit pAb at dilution of 100. Blue: DAPI for nuclear staining.
Alternative Name: ACSS2; ACAS2; ACECS; ACS; ACSA; dJ1161H23.1
Immunogen: Recombinant fusion protein of human ACSS2(Q9NR19).
Conjugate: Unconjugated
Modification: Unmodified