Product Description

Actin (E361) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1002-50, BS1002-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellμlar protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. Alpha Actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regμlate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regμlates Actin filament accumμlation at the plasma membrane and Cdc42 stimμlates formation of filopodia.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: ACTB, Beta-actin, Actin, cytoplasmic 1, β-actin, β actin, βactin, actin-β, actin β, POTEKP, Kappa-actin, ACTBL3, Gamma-actin, ACTG1, ACTG

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids C-terminus of Human Actin.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Actin (E361) polyclonal antibody detects endogenous levels of Actin protein.

Molecular Weight: ~ 45 kDa

Pathway: Regμlation of Actin Dynamics, Regμlation of Actin Dynamics, Contribution of ECM and Cytoskeletal Factors to EMT, Adherens Junction signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P60709/Q9BYX7/P63261

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only