ADAMTS18 Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS60769-50, BS60769-100
Sizes: 50µl, 100µl
Swiss-Prot: Q8TE60
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: ADAMTS (a disintegrin and metalloproteinase domain with Thrombospondin type-1 modules) is a family of zinc-dependent proteases that are implicated in a variety of normal and pathological conditions, including arthritis and cancer. ADAMTS protein family members contain an amino-terminal propeptide domain, a metalloproteinase domain, a disintegrin-like domain and a carboxy-terminus that contains a varying number of thrombospondin 1 (TSP 1) motifs. ADAMTS genes are primarily expressed in fetal tissues, including the lung, kidney and liver. ADAMTS-18 is a secreted protein that is also expressed in adult brain, endothelium, prostate and submaxillary gland. ADAMTS-18 has two known splice variants and it is structurally most similar to ADAMTS-16. ADAMTS-18 is downregulated in carcinoma cell lines by methylation of its promoter. Forced expression of ADAMTS-18 in carcinoma cells can lead to significant inhibition of cell growth, suggesting that it plays a significant role as a tumor suppressor.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: ADAMTS18 polyclonal antibody detects endogenous levels of ADAMTS18 protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ADAMTS18 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate , Immunohistochemistry (IHC) analyzes of ADAMTS18 pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasm staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~ 135 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: A disintegrin and metalloproteinase with thrombospondin motifs 18; ADAM-TS 18; ADAM-TS18; ADAMTS-18; ADAMTS18
Immunogen: A synthetic peptide corresponding to residues in Human ADAMTS18
Conjugate: Unconjugated
Modification: Unmodified