ADAP1 Polyclonal Antibody
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50 µl, 100 µl
Catalogue Numbers: BS72344-50, BS72344-100
Swiss-Prot: O75689
Host: Rabbit
ADAP1 Polyclonal Antibody-BS72344
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: GTPase-activating protein for the ADP ribosylation factor family (Probable. Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3 and inositol 1,3,4,5-tetrakisphosphate (InsP4.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 43kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using ADAP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of U2OS cells using ADAP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Alternative Name: ADAP1; CENTA1; GCS1L; p42IP4
Immunogen: Recombinant fusion protein of human ADAP1(NP_006860.1).
Modification: Unmodified
Conjugate: Unconjugated