ADAR Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90032-50, BS90032-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P55265(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC IF, IHC
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: RNA-specific adenosine deaminase (ADAR1, DSH, IFI4, p136, DRADA, DSRAD, K88dsRBP) mediates RNA editing by destabilizing double stranded RNA through deamination of adenosine to inosine in structured or double-stranded RNAs. ADAR1 is expressed from an interferon-response promoter and has a Z-DNA/Z-RNA binding domain at its N-terminus. ADAR1 co-localizes with SUMO-1 in a subnucleolar region that is distinct from the fibrillar center, the dense fibrillar component and the granular component. Localization of nuclear ADAR1 is under the influence of a nucleolar localization signal (NoLS) in the middle of ADAR1 and the exporting activity of the nuclear exporter signal (NES) near the N terminus. ADAR1 upregulates nuclear factor 90 (NF90)-mediated gene expression by interacting with NF110, NF90 and NF45. ADAR1 binds short interfering RNA (siRNA), and gene silencing by siRNA is significantly more effective in mouse fibroblasts homozygous for an ADAR1 null mutation than in wild-type cells. ADAR1 may limit the efficacy of siRNA in mammalian cells.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: ADAR polyclonal antibody detects endogenous levels of ADAR protein.
Bioworld Molecular Weight: 136 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of ADAR on SiHa cell using anti-ADAR antibody at 1/500 dilution., ICC staining ADAR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: DRADA, p136, IFI-4, ADAR, ADAR1, DSRAD, G1P1, IFI4, 136 kDa double-stranded RNA-binding protein, 136kDa double stranded RNA binding protein, Adar 1, ADAR, Adar1, Adenosine deaminase acting on RNA 1 A, Adenosine deaminase RNA specific 1, Adenosine deaminase RNA specific, Adenosine deaminase that act on RNA, AGS6, AV242451, Double stranded RNA specific adenosine deaminase, Double-stranded RNA-specific adenosine deaminase, Double-stranded RNA-specific editase Adar, DRADA, Dsh, Dsrad, DSRAD, dsRNA adenosine deaminase, EC 3.5.4.-, G1P1, IFI 4, IFI-4, IFI4, Ifi4 protein, Interferon induced protein 4, Interferon inducible protein 4, Interferon-inducible protein 4, K88DSRBP, mZaADAR, P136, Pre-mRNA adenosine deaminase, RNA adenosine deaminase 1, RNA-editing deaminase 1, RNA-editing enzyme 1,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified