Adenosine Deaminase (ADA) Polyclonal Antibody
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50 µl, 100 µl
Catalogue Numbers: BS6958-50, BS6958-100
Swiss-Prot: P00813
Host: Rabbit
Adenosine Deaminase (ADA) Polyclonal Antibody-BS6958
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 41KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using Adenosine Deaminase antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunofluorescence analysis of C6 cells using Adenosine Deaminase ) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Alternative Name: ADA
Immunogen: Recombinant fusion protein of human Adenosine Deaminase (Adenosine Deaminase (ADA))(NP_000013.2).
Modification: Unmodified
Conjugate: Unconjugated