ADH1A Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90034-50, BS90034-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P07327(Human) P00329(Mouse) P06757(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC ICC IF
All Applications: WB:1:500-1:2,000
ICC:1:50-1:100
IHC:1:50-1:200
Background: The alcohol dehydrogenase family of proteins metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha (ADH1A), beta (ADH1B) and gamma (ADH1C) subunits are tandemly organized on chromosome 4q22 as a gene cluster. The alpha form of ADH is monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. The genes encoding beta and gamma subunits, however, are polymorphic and strongly expressed in adult livers. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes. The physiologic function for ADH in the liver is the removal of ethanol formed by microorganisms in the intestinal tract.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: ADH1A polyclonal antibody detects endogenous levels of ADH1A protein.
Bioworld Molecular Weight: 40 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Alcohol Dehydrogenase on rat liver tissue lysate using anti-Alcohol Dehydrogenase antibody at 1/1,000 dilution., ICC staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: ADH1A, ADH1, ADH alpha subunit, ADH, ADH1, ADH1A, ADH1A, Alcohol dehydrogenase 1 (class I), alpha polypeptide, Alcohol dehydrogenase 1, Alcohol dehydrogenase 1A (class I), alpha polypeptide, Alcohol dehydrogenase 1A, Alcohol dehydrogenase subunit alpha, Aldehyde reductase,
Immunogen: Recombinant protein within human Alcohol Dehydrogenase aa 1-200.
Conjugate: Unconjugated
Modification: Unmodified