Adiponectin Polyclonal Antibody
Catalogue Numbers: BS67424-50, BS67424-100
Sizes: 50µl, 100µl
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: Q15848
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: Adiponectin, also termed AdipoQ, Acrp30, apM1 and GBP28, is an adipokine expressed exclusively in brown and white adipocytes . It is secreted into the blood and exists in three major forms: a low molecular weight trimer, a medium molecular weight hexamer and a high molecular weight multimer. Adiponectin levels are decreased in obese and insulin-resistant mice and humans, suggesting that this adipokine is critical to maintain insulin sensitivity. Adiponectin stimulates the phosphorylation of AMPKα at Thr172 and activates AMPK in skeletal muscle. It also stimulates glucose uptake in myocytes . The block of AMPK activation by a dominant-negative AMPKα2 isoform inhibits the effect of adiponectin on glucose uptake, indicating that adiponectin stimulates glucose uptake and increases insulin sensitivity through its action on AMPK . Adiponectin mutants that are not able to form oligomers larger than trimers have no effect on the AMPK pathway . Mutations that render adiponectin unable to form high molecular weight multimers are associated with human diabetes , indicating the importance of multimerization for adiponectin activity.
Purification and Purity: The antibody was purified by immunogen affinity chromatography.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of Adiponectin protein.
Extra Notes: Western blot analysis of Adiponectin expression in mouse heart (A), rat heart (B) whole cell lysates.
Bioworld Molecular Weight: ~ 35 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: ACDC; ACRP30; APM1; GBP28; Adiponectin; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Adiponectin. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified