ADO Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-15460-60, E-AB-15460-120, E-AB-15460-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: ADO
Target Synonym: 2 aminoethanethiol dioxygenase; 2-aminoethanethiol dioxygenase; ADO 2 aminoethanethiol (cysteamine) dioxygenase; ADO; AEDO; C10orf22; Chromosome 10 open reasing frame 22; cysteamine (2-aminoethanethiol) dioxygenase (ADO); Cysteamine dioxygenase; DKFZp564C046; FLJ14547
Research Areas: Metabolism, Signal Transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse
Application: WB, IHC, ELISA
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q96SZ5
Background: Human thiol dioxygenases include cysteine dioxygenase (CDO, MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO, EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.
Concentration: 0.3 mg/mL
Immunogen: Synthetic peptide of human ADO
Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Purification Method: Affinity purification
Dilution: WB 1:1000-1:5000, IHC 1:25-1:100
Calculated MW: 30kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only