AFAP1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS71409-50, BS71409-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q8N556
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:1000 - 1:2000 | IF/ICC,1:50 - 1:200
Background: The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 81kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western Blot analysis of extracts of various cell lines, using AFAP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Alternative Name: AFAP1; AFAP; AFAP-110; AFAP110
Immunogen: Recombinant fusion protein of human AFAP1(NP_940997.1).
Conjugate: Unconjugated
Modification: Unmodified