AFP Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9042-50, MB9042-100
Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P02771(Human)
Host: Mouse
Reactivity: Human
Applications: WB, IHC ICC
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: α-fetoprotein (AFP) is expressed in fetal liver at varying levels throughout development and is present only in trace amounts in normal adult tissues. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker. AFP binds copper, nickel and fatty acids, and in some cases may bind serum albumin or estrogen. It has been demonstrated that the AFP promoter is a target for NF-1 (nuclear factor-1), HNF-1 (hepatocyte nuclear factor-1) and C/EBP transcription factors. While HNF-1 binding to the AFP promoter results in AFP expression, NF-1 binding results in a decrease in AFP promoter activity.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: AFP monoclonal antibody detects endogenous levels of AFP protein.
Bioworld Molecular Weight: 68 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of AFP on human placenta tissue lysate using anti-AFP antibody at 1/1,000 dilution., Western blot analysis of AFP on HepG2 cell lysate using anti-AFP antibody at 1/1,000 dilution.
Alternative Name: HPAFP, AFP, Afp, AFPD, Alpha fetoglobulin, Alpha fetoprotein, Alpha fetoprotein precursor, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein, alpha-fetoprotein, Hereditary persistence of, included, FETA, FETA, Hereditary persistence of alpha fetoprotein, HPAFP,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified