AFP Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90036-50, BS90036-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P02771
Host: Rabbit
Reactivity: Human
Applications: WB, ICC IHC FC
All Applications: WB:1:1,000-1:2,000
ICC:1:200
IHC:1:200
FC:1:100-1:200
Background: AFP is a protein normally produced by the fetal liver and is present in the fluid surrounding the fetus (amniotic fluid), and crosses the placenta into the mother's blood. AFP gene expression is regulated by the interactions between steroid hormone receptors and transcriptional factors in separate signal transduction pathways. AFP functions as a binding and transporting ligand and cell growth regulator. Elevated expression level of AFP has been implicated in colorectal, ovarian, pancreatic, testicular, and certain liver cancers. High levels of AFP are also seen in some diseases such as hepatitis and colitis.
Purification and Purity: Peptide affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: AFP polyclonal antibody detects endogenous levels of AFP protein.
Bioworld Molecular Weight: 70 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of AFP on different cell lysates using anti-AFP antibody at 1/1000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: human lung
Lane 3: human liver, ICC staining AFP in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: AFP, Afp, AFPD, Alpha fetoglobulin, Alpha fetoprotein, Alpha fetoprotein precursor, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein, alpha-fetoprotein, Hereditary persistence of, included, FETA, FETA, Hereditary persistence of alpha fetoprotein, HPAFP,
Immunogen: peptide
Conjugate: Unconjugated
Modification: Unmodified