AGER / RAGE (E182) Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS3448-50, BS3448-100
Sizes: 50µl, 100µl
Swiss-Prot: Q15109
Host: Rabbit
Reactivity: Human
Applications: WB, IHC
Background: Advanced glycosylation end products of proteins (AGEs) are nonenzymatically glycosylated proteins that are associated with a variety of conditions, including diabetes and other vascular disorders, as well as amyloidosis. These proteins regulate cellular functions via specific cell surface acceptor molecules, such as RAGE (receptor for advanced glycosylation end products). RAGE is a type 1 membrane protein that is found on the surface of endothelial cells, mononuclear phagocytes and vascular smooth muscle cells. Binding of AGEs to RAGE results in the induction of cellular oxidant stress and activation of the transcription factor NFkB. Evidence suggests that the induction of oxidant stress results in the activation of an intracellular cascade involving p21 ras and MAP kinase, which leads to activation of transcription.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: AGER (E182) polyclonal antibody detects endogenous levels of AGER protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of RAGE (E182) polyclonal antibody in extracts from HEK293A cells., Immunohistochemistry (IHC) analyzes of AGER / RAGE (E182) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing secreted and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~43 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: Receptor for advanced glycosylation end products; Advanced glycosylation end product-specific receptor; AGER; RAGE
Immunogen: Synthetic peptide, corresponding to amino acids 150-205 of Human AGER / RAGE.
Conjugate: Unconjugated
Modification: Unmodified