AGTR2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90039-50, BS90039-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P50052(Human) P35374(Mouse) Q3US12(Mouse) P35351(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB IP
All Applications: WB:1:1,000-1:2,000
Background: Angiotensin II (Ang II) is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. Although Ang II interacts with two types of cell surface receptors, AT1 and AT2, most of the major cardiovascular effects seem to be mediated through AT1. Molecular cloning of the AT1 protein has shown it to be a member of the G protein-associated seven transmembrane protein receptor family. Ang II treatment of cells results in activation of several signal transduction pathways as evidenced by tyrosine phosphorylation of several proteins and induction of others. PLCγ is phosphorylated after 30 seconds of treatment with Angiotensin II, indicating this as an early signal transduction event. Ang II treatment also stimulates phosphorylation of Shc, FAK and MAP kinases, and induces MKP-1, indicating stimulation of growth factor pathways. Ang II stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by coimmunoprecipitation. The AT1 receptor has no cytoplasmic kinase domain, but is able to function as a substrate for Src kinases and has several putative phosphorylation sites.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of AGTR2 on differentLysates using anti-AGTR2 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Hela
Bioworld Molecular Weight: 41 kDa
Specificity: AGTR2 polyclonal antibody detects endogenous levels of AGTR2 protein.
Alternative Name: AT2, AGTR2, AGTR 2, Agtr2, AGTR2, angiotensin II receptor type 2, Angiotensin II type-2 receptor, Angiotensin receptor 2, AT 2, AT2, ATGR 2, ATGR2, MRX 88, MRX88, Type 2 angiotensin II receptor, Type-2 angiotensin II receptor,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified