AICDA Polyclonal Antibody
Catalogue Numbers: BS60554-50, BS60554-100
Sizes: 50µl, 100µl
Alternative Name: Single-stranded DNA cytosine deaminaseActivation-induced cytidine deaminase; Cytidine a; inohydrolase; AICDA; AID
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9GZX7
Host: Rabbit
Reactivity: Human, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Activation-induced Cytidine Deaminase (AID, HIGM-2) is a 198-amino acid, RNA-editing enzyme that contains a conserved cytidine deaminase motif and plays an important role in B-cell terminal differentiation. AID is expressed in germinal center B cells and contributes to the production of neutralizing antibodies IgG, IgA, and IgE. Hyper-IgM syndrome (HIGM2) patients that have deficient levels of AID show the absence of immuno-globulin class switch recombination (CSR), lack of immuno-globulin somatic hypermutations, and lymph node hyperplasia mediated by the presence of giant germinal centers. Furthermore, AID-/- mice are defective in CSR and also show a hyper-IgM phenotype, characterized by enlarged germinal centers containing active B cells. AID thus appears to be required in several stages of B-cell terminal differentiation that are necessary for efficient antibody responses such as B cell proliferation, immunoglobulin somatic hypermutations and CSR.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: AICDA polyclonal antibody detects endogenous levels of AICDA protein.
Bioworld Molecular Weight: ~ 26 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of AICDA polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human AICDA
Conjugate: Unconjugated
Modification: Unmodified