AIF Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50µl, 100µl
Catalogue Numbers: BS90041-50, BS90041-100
Swiss-Prot: O95831(Human) Q9Z0X1(Mouse) Q9JM53(Rat)
Host: Rabbit
AIF Polyclonal Antibody-BS90041
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC, IP, FC
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: A key event in the apoptotic process is the opening of the mitochondrial permeability transition pore, an event that is regulated by Bcl-2 family proteins, resulting in the release of several proteins from the mitochondrial intermembrane space. Several of these proteins participate in apoptosis, including cytochrome c, procaspases 2, 3, and 9, and AIF (apoptosis-inducing factor). AIF has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIF from mitochondria, but not to block its apoptogenic activity.
Purification and Purity: ProA affinity purified
Specificity: AIF polyclonal antibody detects endogenous levels of AIF protein.
Bioworld Molecular Weight: ~ 67 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of AIF pAb at 1:2000 dilution
Lane 1: Hela whole cell lysate (40ug)
Lane 2: DLD whole cell lysate (40ug)
Lane 3: The Brain tissue lysate of Rat (40ug)
Lane 4: The Brain tissue lysate of Mouse (30ug)
Lane 5: C6 whole cell lysate (40ug)
Lane 6: CT26 whole cell lysate (30ug), ICC staining AIF in Hela cells (green) . The nuclear counter stain is DAPI (blue) . Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: AIFM1, AIFM1, AIFM1, Apoptosis inducing factor 1, mitochondrial, Apoptosis inducing factor, Apoptosis inducing factor, mitochondrion associated, 1, Apoptosis-inducing factor 1, CMTX4, COWCK, COXPD6, Harlequin, Hq, mAIF, MGC111425, MGC5706, mitochondrial, Neuropathy, axonal motor-sensory, with deafness and mental retardation, neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome), PDCD 8, PDCD8, Programmed cell death 8 (apoptosis inducing factor), Programmed cell death 8, Programmed cell death 8 isoform 1, Programmed cell death 8 isoform 2, Programmed cell death 8 isoform 3, Programmed cell death protein 8, Programmed cell death protein 8 mitochondrial, Programmed cell death protein 8 mitochondrial precursor, Programmed cell death protein 8 mitochondrial precursor, Striatal apoptosis inducing factor,
Immunogen: recombinant protein
Modification: Unmodified
Conjugate: Unconjugated