Product Description

AIRE (P126) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1879-50, BS1879-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The autoimmune regμlator gene, which is defective in the hereditary autoimmune disease APECED, encodes the transcriptional activator AIRE. AIRE is expressed in the medμllary epithelial cells and monocyte-dendritic cells of the thymus, with lower expression in the spleen, fetal liver and lymph nodes. In adμlt tissue, AIRE expression in the thymus is confined to the medμlla and the cortico-medμllary junction, where it is modμlated by thymocytes undergoing negative selection. At the cellμlar level, AIRE is located in microtubμlar structures of the cytoskeleton and in discrete nuclear dots resembling ND10 nuclear bodies. AIRE is induced by developing early thymocytes and is associated with the correct establishment of a regμlar thymic environment. AIRE regμlates thymic architecture via transcriptional control of downstream target genes. AIRE mutations in APECED patients may affect thymic T cell selection and the formation of self-tolerance.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: Autoimmune regμlator, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, APECED protein, AIRE, APECED, AIRE1, AIRE-1, APECED protein homolog

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 101-150 of Human AIRE.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: AIRE (P126) polyclonal antibody detects endogenous levels of AIRE protein.

Molecular Weight: ~ 60 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O43918

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only