AKAP 1 (N306) Polyclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Catalogue Numbers: BS2498-50, BS2498-100
Sizes: 50µl, 100µl
Swiss-Prot: Q92667
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Background: AKAP1, also known as AKAP149 in human, AKAP121 in rat, or D-AKAP in mouse is a dual-specificity AKAP which can bind to both RI and RII subunits of PKA with similar affinity. Originally thought to be predominantly restricted to the mitochondria, growing evidence suggests that localization of AKAP1 can be regulated in part by alternative splicing events and that AKAP1 may be present in the endoplasmic reticulum-nuclear envelope membrane network. Peri-nuclear localization, along with the fact that AKAP1 interacts with RNA via one of two nucleotide-binding domains (K homology (KH) and Tudor) have lead some to suggest that AKAP1 may play a role in RNA metabolism. In addition to PKA-RI and -RII, AKAP1 directly interacts with PP1 in a phosphorylation dependent manner and nucleates a complex containing PP2Ac, PKA and RSK1 which modulates RSK1 localization and activity.
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: AKAP 1 (N306) polyclonal antibody detects endogenous levels of AKAP 1 protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of AKAP 1 (N306) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: PC3 whole cell lysate (40ug)
Lane 4: SK-OVCAR3 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of AKAP 1 (N306) pAb in paraffin-embedded human brain tissue.
Bioworld Molecular Weight: ~ 90 to 150 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: A-kinase anchor protein 1, mitochondrial; A-kinase anchor protein 149 kDa; AKAP 149; Dual specificity A-kinase-anchoring protein 1; D-AKAP-1; Protein kinase A-anchoring protein 1; PRKA1; Spermatid A-kinase anchor protein 84; S-AKAP84; AKAP1; AKAP149; PRKA1; AKAP-1; AKAP-149
Immunogen: Synthetic peptide, corresponding to amino acids 274-320 of Human AKAP 1.
Conjugate: Unconjugated
Modification: Unmodified