Product Description

AKAP 1 (N306) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2498-50, BS2498-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: AKAP1, also known as AKAP149 in human, AKAP121 in rat, or D-AKAP in mouse is a dual-specificity AKAP which can bind to both RI and RII subunits of PKA with similar affinity. Originally thought to be predominantly restricted to the mitochondria, growing evidence suggests that localization of AKAP1 can be regμlated in part by alternative splicing events and that AKAP1 may be present in the endoplasmic reticμlum-nuclear envelope membrane network. Peri-nuclear localization, along with the fact that AKAP1 interacts with RNA via one of two nucleotide-binding domains (K homology (KH) and Tudor) have lead some to suggest that AKAP1 may play a role in RNA metabolism. In addition to PKA-RI and -RII, AKAP1 directly interacts with PP1 in a phosphorylation dependent manner and nucleates a complex containing PP2Ac, PKA and RSK1 which modμlates RSK1 localization and activity.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa, AKAP 149, Dual specificity A-kinase-anchoring protein 1, D-AKAP-1, Protein kinase A-anchoring protein 1, PRKA1, Spermatid A-kinase anchor protein 84, S-AKAP84, AKAP1, AKAP149, PRKA1, AKAP-1, AKAP-149

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide, corresponding to amino acids 274-320 of Human AKAP 1.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: AKAP 1 (N306) polyclonal antibody detects endogenous levels of AKAP 1 protein.

Molecular Weight: ~ 130 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q92667

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only