AKAP4 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75967-50, BS75967-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q5JQC9
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Bioworld Molecular Weight: 82KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of various lysates, using AKAP4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of human testis cells using AKAP4 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining., Immunofluorescence analysis of rat testis cells using AKAP4 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.
Alternative Name: AKAP4; AKAP 82; AKAP-4; AKAP82; CT99; FSC1; HI; PRKA4; hAKAP82; p82
Immunogen: Recombinant fusion protein of human AKAP4(NP_003877.2).
Conjugate: Unconjugated
Modification: Unmodified