Product Description

AKAP5 (E7) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1962-50, BS1962-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: AKAPs (A-kinase anchoring proteins), as their name implies, are a family of scaffolding proteins that bind regμlatory subunits of Protein Kinase A (PKA) thus localizing PKA activity to distinct regions of the cell. Beyond a common amphipathic alpha helix that is responsible for recruiting the PKA regμlatory subunit (RIα, RIIα, RIβ, or RIIβ), individual AKAPs contain additional domains responsible for the recrutiment of additional signaling proteins (phosphodiesterases, phosphatases, cytoskeletal components, other kinase, etc.) or restricting AKAP to a specific subcellμlar location. AKAP5 (also known as P75, AKAP75, or AKAP79) is predominantly expressed in neuronal tissues and cells where it serves to localize type II PKA to post-synaptic densities. AKAP5 specifically binds to the regμlatory subunit of PKAIIβ, anchoring the enzyme to the plasma membrane and sites of cytoskeletal/membrane junctions. The other binding domains of AKAP5 have been shown to interact with calmodμlin, PP2B, and calcineurin suggesting that AKAP5 may act to coordinate the cAMP- and Ca2+-sensing pathways in various cell types.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: A kinase (PRKA) anchor protein 5, A kinase anchor protein 5, A kinase anchor protein 79kDa, A-kinase anchor protein 5, A-kinase anchor protein 79 kDa, AKAP 5, AKAP 79, AKAP-5, AKAP5, AKAP75, AKAP79,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human AKAP 5.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: AKAP5 (E7) polyclonal antibody detects endogenous levels of AKAP5 protein.

Molecular Weight: ~ 47, 79 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P24588

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only