AKR1C3 Polyclonal Antibody
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50µl, 100µl
Catalogue Numbers: BS79838-50, BS79838-100
Swiss-Prot: P42330
Host: Rabbit
AKR1C3 Polyclonal Antibody-BS79838
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200
Background: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Unmodification
Bioworld Molecular Weight: 37KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of variousLysates, using AKR1C3 antibody at 1:2510 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunohistochemistry of paraffin-embedded rat heart using AKR1C3 antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Alternative Name: AKR1C3; DD3; DDX; HA1753; HAKRB; HAKRe; HSD17B5; PGFS; hluPGFS
Immunogen: Recombinant fusion protein of human AKR1C3(NP_003730.4).
Modification: Unmodified
Conjugate: Unconjugated