Product Description

AKT (phospho-S473) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0815-50, AP0815-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insμlin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad, forkhead transcription factors, c-Raf, and caspase-9. PTEN phosphatase is a major negative regμlator of the PI3 kinase/Akt signaling pathway. LY294002 is a specific PI3 kinase inhibitor. Another essential Akt function is the regμlation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β. Akt may also play a role in insμlin stimμlation of glucose transport. In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regμlation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 and by negatively regμlating the cyclin dependent kinase inhibitors p27 Kip1 and p21 Waf1/Cip1. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.

Category: Primary Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB, IF, IP

Alternate Names: RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: Synthetic phosphopeptide derived from human AKT around the phosphorylation site of Serine 473.

Dilution: WB: 1:1000~1:2000
IF: 1:100~1:500
IP: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: AKT (phospho-S473) polyclonal antibody detects endogenous levels of AKT protein only when phosphorylated at Ser473.

Molecular Weight: ~ 60 kDa

Pathway: Phosphoinosotode Signaling, PI3K AKT signaling, Protein Kinase C Signaling, Immune Cell Signaling, Warburg Effect, Translational Contral elF4 and p70 S6 Kinase, Regμlation of Microtubμle Dynamics, T cell receptor signaling, Inhibition of Apoptosis, Insμlin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint, Adherens Junction signaling, Mitochondrial Control of Apoptosis, MTOR signaling, ErbB HER Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P31749/P31751/Q9Y243

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only