Product Description

AKT (phospho-S473) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4006-50, BS4006-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regμlatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regμlator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: AKT1, AKT1, AKT2, AKT3, MGC9965, Oncogene AKT1, PKB alpha, PKB, PKB beta, PKBalpha, PRKBA, PRKBB, PRKBG, Protein kinase B, Protein kinase B beta, Protein kinase B gamma, Proto-oncogene c-Akt, RAC alpha, RAC, RAC gamma, RAC PK alpha, RAC PK beta, RAC

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Akt (S473) polyclonal antibody detects endogenous levels of p-Akt protein only when phosphorylated at Ser473.

Molecular Weight: ~ 60 kDa

Pathway: Phosphoinosotode Signaling, PI3K AKT signaling, Protein Kinase C Signaling, Immune Cell Signaling, Warburg Effect, Translational Contral elF4 and p70 S6 Kinase, Regμlation of Microtubμle Dynamics, Regμlation of Microtubμle Dynamics, T cell receptor signaling, Inhibition of Apoptosis, Insμlin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint, Adherens Junction signaling, Mitochondrial Control of Apoptosis, MTOR signaling, ErbB HER Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P31749/P31751/Q9Y243

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only