AKT Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BZ16645-50, BZ16645-100
Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Swiss-Prot: P31749/P31751/Q9Y243
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC/IF, IP, , FC
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 56 kDa; Observed MW: 56 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of AKT in K562, A549 and Jurkat lysates using AKT antibody., Immunohistochemistry analysis of paraffin-embedded Human testis using AKT antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunofluorescence analysis of AKT in MCF-7 using AKT antibody.
Alternative Name: AKT1
Immunogen: A synthesized peptide derived from human AKT1/2/3
Conjugate: Unconjugated
Modification: Unmodified