Akt1 Monoclonal Antibody - BT-MCA0143

Akt1 Monoclonal Antibody

Sizes: 50μL, 100μL

Catalogue Number: BT-MCA0143-50, BT-MCA0143-100

Citations, Manuals and MSDS Available upon request.

Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Research Areas: Cancer; Signal transduction; Metabolism; Epigenetics and nuclear signaling

Target Protein: AKT1

Applications: WB, ELISA

Reactivity: Human,Mouse,Monkey

Clonality: Monoclonal

Host: Mouse

Isotype: IgG

Full Product Name: RAC-alpha serine,threonine-protein kinase

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

UniProt Accession: Human: P31749; Mouse: P31750

Synonyms: AKT1; PKB; RAC; RAC-alpha serine; threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

GeneID: Human: 207; Mouse: 11651

Research Use Only