ALAS1 Rabbit Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS9823M-50, BS9823M-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P13196
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF
All Applications: WB: 1:1000-1:5000
ICC/IF: 1:50-1:200
Background: 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.
Purification and Purity: Protein A affinity purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: This antibody detects endogenous levels of ALAS and does not cross-react with related proteins.
Bioworld Molecular Weight: ~ 71 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ALAS1 Rabbit mAb at 1:1000 dilution
Lane 1: JAR whole cell lysate
Lane 2: HUVEC whole cell lysate
Lane 3: Hela whole cell lysate, ICC staining Alas1 in Hela cells (green). The nuclear counter stain is DAPI (blue).
Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS
Alternative Name: 5-aminolevulinate synthase, nonspecific, mitochondrial; ALAS-H; 5-aminolevulinic acid synthase 1; Delta-ALA synthase 1; Delta-aminolevulinate synthase 1; ALAS1; ALAS3; ALASH; OK/SW-cl.121
Immunogen: Recombinant antibody.
Conjugate: Unconjugated
Modification: Unmodified