Alcohol Dehydrogenase Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75905-50, BS75905-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P07327
Host: Rabbit
Reactivity: Human, Rat
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of various cell lines, using Alcohol Dehydrogenase antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 40kDa
Alternative Name: ADH1A; ADH1
Immunogen: Recombinant fusion protein of human Alcohol Dehydrogenase(NP_000658.1).
Conjugate: Unconjugated
Modification: Unmodified