ALIX / PDCD6IP Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS70704-50, BS70704-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q8WUM4
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:200 - 1:2000 | IF/ICC,1:50 - 1:200
Background: This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 105kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using ALIX / PDCD6IP antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 3min., Immunofluorescence analysis of L929 cells using ALIX / PDCD6IP antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U-251MG cells using ALIX / PDCD6IP antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Alternative Name: PDCD6IP; AIP1; ALIX; DRIP4; HP95
Immunogen: Recombinant fusion protein of human ALIX / PDCD6IP(NP_037506.2).
Conjugate: Unconjugated
Modification: Unmodified