Alkaline Phosphatase Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BZ16603-50, BZ16603-100
Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Swiss-Prot: P05186
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC/IF, IP, , FC
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 57 kDa; Observed MW: 75 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Alkaline Phosphatase in HepG2 lysates; JAR lysates using Alkaline Phosphatase antibody., Immunohistochemistry analysis of paraffin-embedded Human liver using Alkaline Phosphatase antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunofluorescence analysis of Alkaline Phosphatase in Hela using Alkaline Phosphatase antibody., Western blot analysis of Alkaline Phosphatase in SKOV-3, Jurkat and MCF-7 lysates using Alkaline Phosphatase antibody.
Alternative Name: ALPL; Alkaline phosphatase; tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme
Immunogen: A synthesized peptide derived from human Alkaline Phosphatase
Conjugate: Unconjugated
Modification: Unmodified