Product Description

ALKBH8 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS70772-50, BS70772-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: ALKBH8 (alkylated DNA repair protein AlkB homolog 8) is a 664 amino acid protein that is encoded by a gene located on chromosome 11. ALKBH8 contains one RRM (RNA recognition motif) domain and belongs to the AlkB family of proteins. ALKBH8 is one of many homologs of the Escherichia coli protein AlkB. AlkB functions to protect DNA and RNA against damage from environmental methylating compounds by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzyme acts by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate and molecμlar oxygen as the oxidizing agent. Three isoforms exist for ALKBH8 due alternative splicing of the gene.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: ABH8,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant protein of human ALKBH8.

Dilution: WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ALKBH8 polyclonal antibody detects endogenous levels of ALKBH8 protein.

Molecular Weight: ~ 75 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q96BT7

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only