ALKBH8 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS70772-50, BS70772-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q96BT7
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, IHC, IF
All Applications: WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Background: ALKBH8 (alkylated DNA repair protein AlkB homolog 8) is a 664 amino acid protein that is encoded by a gene located on chromosome 11. ALKBH8 contains one RRM (RNA recognition motif) domain and belongs to the AlkB family of proteins. ALKBH8 is one of many homologs of the Escherichia coli protein AlkB. AlkB functions to protect DNA and RNA against damage from environmental methylating compounds by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzyme acts by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate and molecular oxygen as the oxidizing agent. Three isoforms exist for ALKBH8 due alternative splicing of the gene.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ALKBH8 polyclonal antibody detects endogenous levels of ALKBH8 protein.
Bioworld Molecular Weight: ~ 75 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemistry of paraffin-embedded mouse lung using ALKBH8 antibody at dilution of 1:100 (x40 lens)., Immunohistochemistry of paraffin-embedded human gastric cancer using ALKBH8 antibody at dilution of 1:100 (x40 lens)., Immunofluorescence analysis of A549 cell using ALKBH8 antibody. Blue: DAPI for nuclear staining.
Alternative Name: ABH8;
Immunogen: Recombinant protein of human ALKBH8.
Conjugate: Unconjugated
Modification: Unmodified