Alpha-1 Antichymotrypsin (SERPINA3) Polyclonal Antibody
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sizes: 50µl, 100µl
Catalogue Numbers: BS80417-50, BS80417-100
Swiss-Prot: P01011
Host: Rabbit
Alpha-1 Antichymotrypsin (SERPINA3) Polyclonal Antibody-BS80417
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human
Applications: WB, IHC
All Applications: WB,1:200 - 1:1000 | IHC,1:50 - 1:200
Background: The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Unmodification
Bioworld Molecular Weight: 50-70KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of Human plasma, using Alpha-1 Antichymotrypsin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunohistochemistry of paraffin-embedded human liver cancer using Alpha-1 Antichymotrypsin ) antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Alternative Name: SERPINA3; AACT; ACT; GIG24; GIG25
Immunogen: A synthetic peptide of human Alpha-1 Antichymotrypsin (SERPINA3)(NP_001076.2).
Modification: Unmodified
Conjugate: Unconjugated