Alpha-actin-1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB65868-50, MB65868-100
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: P68133
Host: Mouse
Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IP,
All Applications: WB (1/5000 - 1/10000), IHC (1/200 - 1/500), IP (1/100 - 1/200)
Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of Alpha-actin-1 protein.
Bioworld Molecular Weight: ~ 43 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Alpha-actin-1 expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates., Immunohistochemical analysis of Alpha-actin-1 staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunoprecipitation of Alpha-actin-1 from 0.5mg mouse brain whole cell extract lysate, using Anti-Alpha-actin-1 Antibody.
Alternative Name: ACTA; Actin alpha skeletal muscle; Alpha-actin-1
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Alpha-actin-1. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified