Product Description

Alpha-Glucosidase Inhibitor Screening Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-D017-48, E-BC-D017-96

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Category: Cell Metabolism Assays

Storage and Stability: This product can be stored at -20°C for 12 months with shading light.

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Inhibition rate

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Tested Sample Types: Inhibitor

Assay Length: 30 min

Test Principle: Alpha-glucosidase catalyzes the release of α-glucose from the non-reducing end of the substrate and promotes the absorption of glucose by the small intestine. Inhibition of α-glucosidase is an effective method for the treatment of non-insulin-dependent diabetes mellitus (NIDDM).
The detection principle of this kit: α-Glucosidase catalyzes the substrate reaction to generate colored products, whose absorbance increases at 400 nm. The addition of an inhibitor suppresses the enzymatic activity of α-glucosidase, resulting in a reduced rate of absorbance increase. The inhibition rate can be calculated based on the absorbance difference.

Inter CV: 5.6-8.6%

Intra CV: 0.2-2.0%

Research Use Only