Alpha-tubulin (AcK40) Monoclonal Antibody
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Catalogue Numbers: MB65872-50, MB65872-100
Sizes: 50µl, 100µl
Swiss-Prot: P68363
Host: Mouse
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Recognizes endogenous levels of Alpha-tubulin (AcK40) protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Alpha-tubulin (AcK40) expression in HeLa (A) whole cell lysates., Immunohistochemical analysis of Alpha-tubulin (AcK40) staining in human breast cancer,mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Bioworld Molecular Weight: ~ 54 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Alpha-tubulin. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified