ALPP Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90049-50, BS90049-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P05187(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Alkaline phosphatases (AP) are glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+-metallated glycoproteins that catalyze the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. Placental alkaline phosphatase (also known as PLAP, ALPP, PALP, placental ALP-1 or Regan isozyme) is a 530 amino acid, tissue-specific AP that is expressed in the placenta, the serum of pregnant women and ectopically expressed in various cancers, including those of the ovary and testis. PLAP may assist in guiding migratory cells and transporting specific molecules, such as fatty acids and immunoglobulins, across the plasma membrane. The three tissue-specific APs identified in human, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous and their genes are clustered on chromosome 2q.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: ALPP polyclonal antibody detects endogenous levels of ALPP protein.
Bioworld Molecular Weight: 70 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PLAP on human placenta tissue (1) and Hela cell (2) lysate using anti-PLAP antibody at 1/1,000 dilution., ICC staining PLAP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: PLAP-1, ALPP, PLAP, Alkaline phosphatase, Alkaline phosphatase placental, Alkaline phosphatase placental type, Alkaline phosphatase Regan isozyme, ALP, Alp1, ALPP, FLJ61142, Germ-cell alkaline phosphatase, nagao isozyme, OTTHUMP00000164354, PALP, Placental alkaline phosphatase 1, placental heat-stable alkaline phosphatase, placental type, PLAP, PLAP-1, PLAP1, PPB1,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified