AMD1 Polyclonal Antibody
Catalogue Numbers: BS60408-50, BS60408-100
Sizes: 50µl, 100µl
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P17707
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB
All Applications: WB: 1:500~1:1000
Background: AdoMetDC (adenosylmethionine decarboxylase 1), also known as S-adenosylmethionine decarboxylase proenzyme (SAMDC) or AMD1, is a 334 amino acid protein which is an important intermediate enzyme in polyamine biosynthesis pathways. Using a pyruvoyl group as a cofactor, AdoMetDC catalyzes the conversion of S-adenosyl-L-methionine to (5-deoxy-5-adenosyl)(3-aminopropyl)-methylsulfonium salt and carbon dioxide. AdoMetDC is synthesized as an inactive proenzyme that undergoes self-maturation to form two non-identical subunits designated α and β. Active AdoMetDC forms a heterotetramer of two α chains and two β chains. Both AdoMetDC proenzyme processing and mature AdoMetDC catalytic activity are stimulated by putrescine, while catalytic activity is inhibited by iodoacetic acid.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: AMD1 polyclonal antibody detects endogenous levels of AMD1 protein.
Extra Notes: Western blot (WB) analysis of AMD1 pAb at 1:500 dilution
Lane1:AML-12 whole cell lysate(40ug)
Lane2:HCT116 whole cell lysate(40ug)
Lane3:L02 whole cell lysate(40ug)
Lane4:HepG2 whole cell lysate(40ug)
Lane5:A549 whole cell lysate(40ug)
Bioworld Molecular Weight: ~ 38 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: S-adenosylmethionine decarboxylase proenzyme; AdoMetDC; SAMDC; S-adenosylmethionine decarboxylase alpha chain; S-adenosylmethionine decarboxylase beta chain; AMD
Immunogen: A synthetic peptide corresponding to residues in Human AMD1.
Conjugate: Unconjugated
Modification: Unmodified