Product Description

AMPKα1/2 (T174/T172) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0569-50, AP0569-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regμlation of energy homeostasis. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regμlatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2, β1, 2, γ1, 2, 3). The kinase is activated by an elevated AMP/ATP ratio due to cellμlar and environmental stress, such as heat shock, hypoxia, and ischemia. The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation. AMPKα is also phosphorylated at Thr258 and Ser485 (for α1, Ser491 for α2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated. The β1 subunit is post-translationally modified by myristoylation and mμlti-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182. Phosphorylation at Ser108 of the β1 subunit seems to be required for AMPK activation, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization. Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumμlation in heart or skeletal muscle. Accumμlating evidence indicates that AMPK not only regμlates the metabolism of fatty acids and glycogen, but also modμlates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS.

Category: Primary Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB

Alternate Names: 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA1, AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2, AMPK, AMPK2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to Human AMPKα1/2.

Dilution: WB: 1:2000~1:5000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: AMPKα1/2 (T174/T172) polyclonal antibody detects endogenous levels of AMPKα1/2 protein.

Molecular Weight: ~ 65 kDa

Pathway: Hypoxia Signaling, Warburg Effect, Translational Contral elF4 and p70 S6 Kinase, Insμlin &Glucose Signaling, AMPK Signaling Pathway, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13131/P54646

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only