Product Description

AMPK alpha 1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67087-50, MB67087-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regμlation of energy homeostasis. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regμlatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2, β1, 2, γ1, 2, 3). The kinase is activated by an elevated AMP/ATP ratio due to cellμlar and environmental stress, such as heat shock, hypoxia, and ischemia. The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation. AMPKα is also phosphorylated at Thr258 and Ser485 (for α1, Ser491 for α2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated. The β1 subunit is post-translationally modified by myristoylation and mμlti-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182. Phosphorylation at Ser108 of the β1 subunit seems to be required for AMPK activation, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization. Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumμlation in heart or skeletal muscle. Accumμlating evidence indicates that AMPK not only regμlates the metabolism of fatty acids and glycogen, but also modμlates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC

Alternate Names: AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human AMPK alpha 1. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Specificity: Recognizes endogenous levels of AMPK alpha 1 protein.

Molecular Weight: ~ 62 kDa

Pathway: MTOR signaling，Warburg Effect，Autophagy Signaling，AMPK Signaling Pathway

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13131

Product: Mouse IgG1 kappa. Supplied in crude ascites with 0.01% sodium azide.

Research Use Only