AMPK alpha1/2 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-30491-60, E-AB-30491-120, E-AB-30491-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: AMPKα1/2
Target Synonym: PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;
Research Areas: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat, Monkey
Application: WB, IHC-p, ELISA
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q13131,P54646
Background: AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.
Concentration: 1 mg/mL
Immunogen: Synthesized peptide derived from human AMPKα1/2 around the non-phosphorylation site of Thr183/172.
Buffer: PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:40000
Calculated MW: 62kDa
Observed MW: 63kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only