AMPK beta 1 Monoclonal Antibody
Catalogue Numbers: MB67259-50, MB67259-100
Sizes: 50µl, 100µl
Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: Q9R078/P80386
Host: Mouse
Reactivity: Mouse, Rat
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) (By similarity).
Purification and Purity: This antibody is purified through a protein G column.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of AMPK beta 1 protein.
Extra Notes: Western blot analysis of AMPK beta 1 expression in C2C12 (A), H4IIE (B), NIH3T3 (C), PC12 (D) whole cell lysates.
Bioworld Molecular Weight: ~ 40 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: AMPK; 5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb
Immunogen: Recombinant fusion protein of human AMPK beta 1. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified