Amylin (H28) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS1381-50, BS1381-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P10997
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: IHC, IF
All Applications: IHC: 1:50~1:200
IF: 1:50~1:200
Background: Adrenomedullin (AM), alpha- and beta-calcitonin gene-related peptide(CGRP), calcitonin (CT), and amylin are homologous polypeptides with overlapping biological actions such as vasodilatation and inhibition of bone resorption. Amylin (Islet/insulinoma amyloid polypeptide or IAPP) is a 37-amino acid monomeric polypeptide isolated from pancreatic amyloid. This protein, with a calculated molecular mass of 4 kDa, is a major component of amyloid-rich pancreatic extracts of three type 2 diabetic patients. IAPP has cysteine residues in positions 2 and 7, a feature found in all known calcitonin gene-related peptides. IAPP shows 46% amino acid sequence homology with CGRP II. Since IAPP has been demonstrated immunochemically in normal beta cells of several mammals, it probably has an important role in respect to pancreatic islet function.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Amylin (H28) polyclonal antibody detects endogenous levels of Amylin protein.
Bioworld Molecular Weight: ~ 10 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemistry (IHC) analyzes of Amylin (H28) pAb in paraffin-embedded mouse brain tissue at 1:50, showing distinct cytoplasmic membrane and secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Amylin (H28) pAb in paraffin-embedded human colon carcinoma tissue at 1:50, showing distinct cytoplasmic membrane and secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: Islet amyloid polypeptide; Amylin; Diabetes-associated peptide; DAP; Insulinoma amyloid peptide; IAPP
Immunogen: Synthetic peptide, corresponding to amino acids 1-50 of Human Amylin.
Conjugate: Unconjugated
Modification: Unmodified