Product Description

ANAPC5 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS61681-50, BS61681-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The anaphase-promoting complex (APC) is composed of more than ten subunits, including APC1, APC2, APC4, APC5, APC7, APC8, APC10 and APC11. The APC acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regμlatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regμlatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate APC’s cyclin-ubiquitination activity. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC1, previously referred to as Tsg24 in mice, is the largest of the APC subunits and is encoded by a gene mapping to 2q12.1.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB

Alternate Names: Anaphase-promoting complex subunit 5, APC5, Cyclosome subunit 5, ANAPC5, APC5

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding Human ANAPC5.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: ANAPC5 polyclonal antibody detects endogenous levels of ANAPC5 protein.

Molecular Weight: ~ 85 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9UJX4

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only